Apparatuses and compositions for cryopreservation of...

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01) C12M 3/04 (2006.01) C12N 1/04 (2006.01)

Patent

CA 2760175

Provided are apparatuses for cryopreserving cells which include a vessel comprising a biocompatible substrate, wherein the vessel further comprises an interior and an exterior, and a mechanical ice nucleating device disposed in or on the vessel interior for initiating ice crystal formation. Also provided are kits comprising one or more apparatuses for cryopreserving cells and a biopreservation medium. Further provided are compositions comprising a vessel for holding cells, a mechanical ice nucleating device, a biopreservation medium, and cells disposed in the vessel. The apparatuses, kits, and compositions of the invention can optionally include an insulating material which is disposed on all or a portion of the vessel.

L'invention porte sur des appareils pour cryoconserver des cellules qui comprennent un récipient comprenant un substrat biocompatible, le récipient comprenant en outre un intérieur et un extérieur, et un dispositif de nucléation de la glace mécanique disposé dans ou sur l'intérieur de récipient pour initier une formation de cristaux de glace. L'invention porte également sur des coffrets comprenant un ou plusieurs appareils pour cryoconserver des cellules et sur un milieu de bioconservation. L'invention porte en outre sur des compositions comprenant un récipient pour contenir des cellules, un dispositif de nucléation de la glace mécanique, un milieu de bioconservation et des cellules disposées dans le récipient. Les appareils, coffrets et compositions de l'invention peuvent facultativement comprendre un matériau isolant qui est disposé sur tout ou partie du récipient.

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