C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
195/141, 23/434,
C12M 1/40 (2006.01) B01J 19/00 (2006.01) B01L 7/00 (2006.01) C07H 21/00 (2006.01) C12M 1/38 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) G05D 23/19 (2006.01)
Patent
CA 1339653
There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.
528641
Johnson Larry J.
Leath Richard A.
Mezei Louis M.
Mullis Kary B.
Wennberg Thimothy J.
Borden Ladner Gervais Llp
Cetus Corporation
Perkin-Elmer Corporation
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