Application of protein - protein interaction as an assay for...

Details Application of protein - protein interaction as an assay for... Application of protein - protein interaction as an assay for...

G - Physics

01

N

195/33

G01N 33/60 (2006.01) C12N 9/00 (2006.01) C12Q 1/25 (2006.01)

Patent

CA 1094476

ABSTRACT OF THE DISCLOSURE A specific type of protein, which has been designated B-protein (Bucovaz-protein), is present in most serum of indivi- duals with cancer. The B-protein interacts with a low molecular weight protein component, which is released by the coenzyme A- synthesizing protein complex (Coa-SPC) of Bakers' yeast during the course of CoA synthesis. This low molecular weight protein, for purposes of identification, is referred to as binding protein. The binding protein has a molecular weight of 10,000 to 15,000. Interaction of radioactively labeled binding protein of CoA-SPC with the B-protein of serum provides a marker for the detection of cancer. Of 3005 serums assayed, which included 908 patients diagnosed as having cancer, the B-protein assay agreed with the clinical diagnosis in more than 89% of the cases.

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