Application of shear flow stress to chondrocytes

Details Application of shear flow stress to chondrocytes Application of shear flow stress to chondrocytes

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12M 3/00 (2006.01) C12M 3/02 (2006.01) C12M 3/04 (2006.01) C12N 5/06 (2006.01) C12N 11/00 (2006.01) C12N 11/02 (2006.01)

Patent

CA 2272547

A bioreactor and method are provided for application of shear flow stress of about 1 to about 100 dynes/cm2 to cultured mammalian cells used for production of artificial cartilage. The bioreactor can be a circulating flow system (16) containing a media reservoir (9), a pump (10), a growth chamber (11) containing a substrate such as a monolayer supporting surface or a 3- dimensional scaffold, and tubing (12). Shear flow stress can be applied by growing a chondrocyte monolayer on the surface of a rotating drum or rotating disc immersed in liquid growth medium, by growing a chondrocyte monolayer on static plates past which a liquid growth medium is pumped, or by establishing chondrocytes in a 3-dimensional scafold and pumping liquid growth medium through the scaffold. Application of flow shear stress increases the ratio of type II to type I cartilage produced by cultured chondrocytes, and enhances maintenance of chondrocyte phenotype.

L'invention concerne un bioréacteur et un procédé destinés à appliquer une contrainte par courant de cisaillement, d'environ 1 à environ 100 dynes/cm?2¿, à des cellules mammaliennes en culture utilisées dans la production de cartilage artificiel. le bioréacteur peut consister en un système (16) de courant circulant comprenant un réservoir (9) de milieux, une pompe (10), une chambre de croissance (11) contenant un substrat tel qu'une surface portant une couche simple ou une construction tridimensionnelle, et un tube (12). On peut appliquer la contrainte par courant de cisaillement en développant une couche simple de chondrocytes à la surface d'un tambour rotatif ou d'un disque rotatif immergé dans un milieu de croissance liquide, en développant une couche simple de chondrocytes sur des plaques statiques à travers lesquelles on pompe un milieu de croissance liquide, ou en plaçant des chondrocytes dans une construction tridimensionnelle, à travers laquelle on pompe le milieu de croissance liquide. Par application de la contrainte par courant de cisaillement, on augmente le rapport du cartilage de type II au cartilage de type I produit par des chondrocytes en culture, et on améliore le maintien du phénotype du chondrocyte.

Affiliated with

Also associated with

Rating

Application of shear flow stress to chondrocytes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Application of shear flow stress to chondrocytes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Application of shear flow stress to chondrocytes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1646102