Aptamers containing sequences of nucleic acids or nucleic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 19/00 (2006.01) C07H 21/02 (2006.01) C12N 15/11 (2006.01) C12P 19/34 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2461526

An aptamer contains at least two parallel or antiparallel heteropolymeric nucleobase-containing sequences bonded together by Watson-Crick complementary base interaction or by homologous base interaction, provided that: (a) when the aptamer is single-stranded, the at least two sequences are bonded together by homologous base interaction; and (b) when the aptamer is a duplex and the at least two sequences are antiparallel to each other, the at least two sequences are bonded together by homologous base interaction. The aptamer can be used to bind ligands or to catalyze reactions when functioning as an aptazyme.

L'invention concerne un aptamère contenant au moins deux séquences parallèles ou antiparallèles qui contiennent des nucléobases hétéropolymères et sont liées par une interaction de bases complémentaires de type Watson-Crick ou par une interaction de bases homologues, à condition que: (a) lorsque l'aptamère est monocaténaire, lesdites séquences sont liées par une interaction de bases homologues ; et (b) lorsque l'aptamère est un duplex et que lesdites séquences sont antiparallèles, lesdits séquences sont liées par une interaction de bases homologues. L'aptamère peut être utilisé pour lier des ligands ou catalyser des réactions lorsqu'il fonctionne comme une aptazyme.

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