C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) A61K 31/70 (2006.01) A61K 39/385 (2006.01) A61K 39/39 (2006.01) A61K 47/48 (2006.01) C07H 21/00 (2006.01) C07K 1/22 (2006.01) C12N 9/00 (2006.01) C12N 9/99 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2104698
2104698 9214843 PCTABS00015 A method for identifying oligomer sequences which specifically bind target molecules such as serum proteins, kinins, eicosanoids and extracellular proteins is described. The method is used to generate aptamers that bind to serum Factor X, thrombin, bradykinin, PGF2.alpha. and cell surface molecules. The technique involves complexation of the target molecule with a mixture of oligonucleotides containing random sequences and sequences which serve as primer for PCR under conditions wherein a complex is formed with the specifically binding sequences, but not with the other members of the oligonucleotide mixture. The complex is then separated from uncomplexed oligonucleotides and the complexed members of the oligonucleotide mixture are recovered from the separated complex using the polymerase chain reaction. The recovered oligonucleotides may be sequenced, and successive rounds of selection using complexation, separation, amplification and recovery can be employed. The oligonucleotides can be used for therapeutic and diagnostic purposes.
Bock Louis C.
Griffin Linda C.
Krawczyk Steven
Latham John A.
Muenchau Daryl Dean
Borden Ladner Gervais Llp
Gilead Sciences Inc.
Gilead Sciences Inc.
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