Arabinogalactan protein compositions and methods for...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) C12N 15/29 (2006.01)

Patent

CA 2560944

Methods for fostering somatic embryogenic competence of a plant cell or tissue by contacting the plant cell or tissue with an arabinogalactan protein (AGP) composition effective for fostering somatic embryogenic competence in plant species and varieties that are recalcitrant to somatic embryogenesis and/or regeneration are provided. AGP compositions useful for fostering somatic embryogenic competence are provided, including total AGP and an AGP RP-HPLC hydrophobic fraction, both from embryogenic callus, from plant varieties including cotton lines Coker 315, Siokra 1-4, and Sicala 40, including at concentrations ranging from about 0.0008 mg/L to about 100 mg/L. Methods for regenerating plants, producing transformed plants, and impeding somatic embryogenic competence are also provided. Plant culture media and methods for making useful AGP compositions and media are also provided. Amino acid and nucleotide sequences for peptides and proteins eluting in hydrophobic AGP fractions are also provided.

Des procédés d'accueil d'une compétence embryogène somatique d'une cellule végétale ou d'un tissu végétal par la mise en contact de la cellule ou du tissu végétal(e) avec une composition protéique d'arabinogalactane (AGP) efficace pour accueillir la compétence embryogène somatique dans les espèces et les variétés végétales résistant à l'embryogenèse et/ou la régénération somatiques. Des compositions AGP sont utilisées pour accueillir la compétence embryogène somatique y compris l'AGP totale et une fraction hydrophobe AGP RP-HPLC, à partir d'un calle embryogène, provenant de variétés végétales comprenant des amarres en coton Coker 315, Siokra 1-4, et Sicala 40, y compris à des niveaux de concentration allant d'environ 0,0008 mg/L à environ 100 mg/L. des procédés de régénération de plantes, de production de plantes transgéniques et l'empêchement de la compétence embryogène somatique. Des milieux de culture de plantes et des procédés d'obtention de compositions AGP et de milieux utiles. Des séquences d'acides aminés et de nucléotides pour des peptides et des protéines s'éluant dans des fractions AGP hydrophobes.

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