Arachnocampa luciferases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/02 (2006.01) C12N 15/09 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2619686

Nucleotide and amino acid sequences of luciferase peptides that are encoded by genes within the genome of Arachnocampa (Diptera) are disclosed. Specifically provided are functional ATP-dependent luciferases that catalyze luminescence reactions with emission spectra within the blue portion of the spectrum. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and active subsequences of the enzyme peptides, and methods of identifying modulators and substrates of the luciferase peptides. Methods of assays, including multiple reporter assays utilizing at least two ATP-dependent luciferases are provided.

La présente invention concerne des séquences d'acides aminés et de nucléotides de peptides de luciférase codés par des gènes dans le génome d'Arachnocampa (Diptera). Elle concerne notamment des luciférases fonctionnelles dépendantes d'ATP qui catalysent des réactions de luminescence avec des spectres d'émission dans la partie bleue du spectre. L'invention concerne plus précisément des molécules d'acides nucléiques et des peptides isolés, des procédés d'identification d'orthologues et de sous-séquences actives de peptides enzymatiques, ainsi que des procédés d'identification de modulateurs et de substrats de peptides de luciférase. L'invention concerne enfin des procédés d'essais, y compris plusieurs essais par reporteurs utilisant deux ou plusieurs luciférases dépendantes d'ATP.

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