Arginine wash in protein purification using affinity...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/22 (2006.01) A61K 39/395 (2006.01) B01D 15/08 (2006.01) C07K 1/14 (2006.01) C07K 16/00 (2006.01)

Patent

CA 2661645

The invention relates to methods for isolating a product and/or reducing turbidity and/or impurities from a load fluid comprising the product and one or more impurities by passing the load fluid through a medium, followed by at least one wash solution comprising arginine or an arginine derivative, and collecting the product using an elution solution. The invention further relates to a product prepared using a method as described herein.

Cette invention concerne des procédés permettant d'isoler un produit et/ou de réduire la turbidité et/ou la teneur en impuretés d'un fluide de charge contenant le produit et une ou plusieurs impuretés, lesquels procédés consistent à faire passer à travers un milieu ledit fluide de charge suivi d'au moins une solution de lavage contenant de l'arginine ou un dérivé d'arginine, puis à recueillir le produit au moyen d'une solution d'élution. Cette invention concerne également un produit préparé au moyen d'un procédé ci-décrit.

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