Array and method for analysing nucleic acid sequences

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01)

Patent

CA 2352476

The invention relates to an array for analysing a nucleic acid sequence or mixture of nucleic acid sequences, comprising: a) a carrier; and b) at least two different nucleic acid sequences bound to said carrier, in which each of the nucleic acid sequences bound to the carrier comprises at least a nucleic acid sequence that corresponds to the sequence of a restriction fragment obtainable by restricting a genomic DNA and/or at least one cDNA with at least one frequent cutter restriction enzyme and at least one rare cutter restriction enzyme. The array preferably comprises at least 10, preferably at least 100, more preferably at least 1000 different nucleic acid sequences bound to the carrier. More preferably, the array comprises a plurality of AFLP~-markers taken from a single individual or from a group of related individuals. The invention also relates to a method for providing nucleic acid sequences, and in particular AFLP~-markers, for use in such an array, as well as to a method for analysing a nucleic acid sequence or a mixture of nucleic acids sequences using such an array. Finally, the invention relates to a kit of parts comprising an array of the invention, as well as to data obtained using the array of the invention.

L'invention concerne un arrangement servant à analyser une séquence d'acides nucléiques ou un mélange de séquences d'acides nucléiques. Cet arrangement comprend : a) un support, et b) au moins deux séquences d'acides nucléiques différentes, liées audit support, chaque séquence liée comprenant au moins une séquence d'acides nucléiques correspondant à la séquence d'un fragment de restriction que l'on peut obtenir par restriction d'un ADN génomique et/ou d'au moins un ADNc avec au moins une enzyme de restriction à points de coupure fréquents et au moins une enzyme de restriction à point de coupure rare. Cet arrangement comprend, de préférence, au moins 10, préférablement au moins 100, et idéalement au moins 1000 séquences d'acides nucléiques différentes liées au support. En outre, cet arrangement comprend plusieurs marqueurs AFLP~ pris à partir d'un individu ou d'un groupe d'individus apparentés. L'invention concerne également un procédé de préparation de séquences d'acides nucléiques, et notamment de marqueurs AFLP~, utiles dans un tel arrangement, de même que des procédés d'analyse d'une séquence d'acides nucléiques ou d'un mélange de séquences d'acides nucléiques au moyen d'un tel arrangement. Enfin, l'invention concerne une trousse d'éléments comprenant notamment l'arrangement de l'invention, de même que des données obtenues au moyen de cet arrangement.

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