Arrays comprising a fiducial and automated information...

B - Operations – Transporting – 01 – J

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B01J 19/00 (2006.01) G01N 21/64 (2006.01) G01N 21/77 (2006.01)

Patent

CA 2359352

The present invention provides array compositions comprising a substrate with a surface comprising discrete sites, at least one fiducial, and a population of microspheres comprising at least a first and a second subpopulation, each comprising a bioactive agent, and distributed on said surface. Each subpopulation may optionally comprise a unique optical signature, an identifier binding ligand, or both. The invention further provides compositions comprising a computer readable memory, comprising data acquisition, registration, and comparison modules to direct a computer to function in a specified manner. The registration module may utilize any number of fiducials, including a fiducial fiber, a fiducial microsphere, or a fiducial template generated from the random array. In further aspects, the invention provided methods of making the array compositions of the invention, for comparing separate data images of a random array, for decoding a random array composition, and for determining the presence of a target analyte in a sample.

L'invention a trait à l'utilisation d'un système informatique pour comparer des images produites par un groupement ordonné de manière aléatoire. Ce système conserve la position relative de chaque site à l'intérieur du groupement de façon à permettre une comparaison d'un même site dans des images différentes.

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