Arrays of biological membranes and methods and use thereof

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 17/00 (2006.01) B01J 19/00 (2006.01) C07K 14/705 (2006.01) C07K 17/06 (2006.01) C07K 17/08 (2006.01) C07K 17/14 (2006.01) C12N 11/02 (2006.01) C12N 11/06 (2006.01) G01N 27/00 (2006.01) G01N 27/26 (2006.01) G01N 33/53 (2006.01) G01N 33/553 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2447251

The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase.

La présente invention permet de pallier les problèmes et les désavantages associés aux réseaux de la technique antérieure. Elle se rapporte à un réseau comprenant une pluralité de microspots de membranes biologiques associés à une surface d'un substrat pouvant être produit, utilisé et stocké non pas dans un milieu aqueux mais dans un milieu exposé à l'air présentant une humidité ambiante ou régulée. De préférence, ces microspots de membranes biologiques comprennent une protéine liée à la membrane. De préférence encore, cette protéine liée à la membrane est un récepteur couplé aux protéines G, un canal ionique, une sérine/thréonine kinase récepteur ou une tyrosine kinase récepteur.

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