Artificial antibody polypeptides

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07K 14/78 (2006.01) C07K 16/00 (2006.01) C12N 1/21 (2006.01) C12N 15/10 (2006.01) C12N 15/70 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2416219

The present invention provides a fibronectin type III(Fn3) molecule, wherein the Fn3 contains a stabilizing mutation. The present invention also provides Fn3 polypeptide monobodies, nucleic acid molecules encoding monobodies, and variegated nucleic acid molecules encoding monobodies, and variegated nucleic acid libraries encoding such monobodies. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform the methods.

La présente invention concerne une molécule de fibronectine de type III (Fn3), la Fn3 comprenant une mutation de stabilisation. La présente invention concerne également des monocorps de polypeptide Fn3, des molécules d'acides nucléiques codant pour ces monocorps, et des banques d'acides nucléiques panachés codant pour ces monocorps. L'invention concerne également des procédés permettant de préparer un monocorps de polypeptide Fn3, ainsi que des trousses permettant de réaliser ces procédés.

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