Artificial chromosome

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) C12N 1/19 (2006.01) C12N 9/12 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2265939

An artificial mammalian chromosome, more specifically, a clone containing a mammalian centromere sequence and a DNA replication origin and having mammalian telomere sequences added to both ends is provided by preparing a library of CEPH artificial yeast chromosomes containing a human genome inserted thereinto, identifying clones holding a repetitive human alphoid sequence from this library, and further preparing a yeast strain which adds mammalian telomere sequences to the ends of the chromosome.

L'invention porte sur un chromosome artificiel de mammifère et plus spécifiquement sur un clone contenant une séquence de centromère de mammifère et une origine de réplication d'ADN, et présentant des séquences de télomère de mammifère ajoutées à ses deux extrémités. Ledit chromosome est obtenu par préparation d'une bibliothèque de chromosomes CEPH de levure artificielle où est inséré un génome humain; par identification des clones de cette bibliothèque présentant une séquence alphoïde humaine répétitive, puis par préparation d'une souche de levure qui ajoute les séquences de télomère de mammifère aux extrémités du chromosome.

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