Artificial chromosomes, uses thereof and methods for...

Details Artificial chromosomes, uses thereof and methods for... Artificial chromosomes, uses thereof and methods for...

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) A61K 31/711 (2006.01) A61K 48/00 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/82 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2429724

Methods for preparing cell fines that contain artificial chromosomes, methods for preparation of artifcial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, and methods for delivery of the chromosomes to selected cells and tissues are provided. Also provided are cell lines for use in the methods, and cell lines and chromosomes produced by the methods. In particular, satellite artificial chromosomes that, except for inserted heterologous DNA, are substantially composed of heterochromatin, are provided. Methods for use of the artificial chromosomes, including f~r gene therapy, production of gene products and production of transgenic plants and animals are also provided.

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