Artificial gene coding for authentic human serum albumin,...

C - Chemistry – Metallurgy – 12 – N

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530/13, 195/1.22

C12N 15/14 (2006.01) C07K 14/765 (2006.01) C12N 15/66 (2006.01) C12N 15/81 (2006.01)

Patent

CA 1340650

A structural gene coding for authentic human serum albumin, - optionally supplemented by an upstream triplet coding for methionine and optionally extended by a synthetic prepro X-leader-coding sequence - , wherein the codons of the nucleotide sequence have been selected with regard to a non-human host, e.g. yeast, chosen for expression of authentic human serum albumin, is disclosed. Additionally there is disclosed a method of producing said gene. There are also disclosed a recombinant DNA molecule comprising said strucural gene inserted into a vector, and a host transformed with said recombinant DNA molecule. Furthermore there are disclosed a method of producing authentic human serum albumin, an authentic human serum albumin resulting from said method, and a pharmaceutical composition comprising said resulting-human serum albumin.

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