Artificial genes for use as controls in gene expression...

Details Artificial genes for use as controls in gene expression... Artificial genes for use as controls in gene expression...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2464839

Method of producing universal controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from intergenic or intronic regions of a genome. Twenty-three specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

Cette invention concerne une méthode d'obtention de témoins universels utilisés dans le cadre de systèmes d'analyse de l'expression génique tels que jeux ordonnés de macro-échantillons, réactions en chaîne de la polymérase en temps réel, transferts de Northern, méthode SAGE et microtitrations. Les témoins sont obtenus soit à partir de séquences quasi-aléatoires d'ADN ou de régions intergéniques ou introniques d'un génome. L'invention porte en outre sur vingt-trois séquences témoins spécifiques. Sont également décrites des méthodes d'utilisation de ces témoins, notamment comme témoins négatifs, comme témoins positifs et comme étalons pour un système d'analyse d'expression génique.

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