Ascorbate interference-resistant composition and method of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/28 (2006.01) C12M 1/40 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2067866

ASCORBATE INTERFERENCE-RESISTANT COMPOSITION, DEVICE AND METHOD OF ASSAYING FOR A PREDETERMINED ANALYTE Abstract of the Disclosure A new and improved test device and method of determining the presence or concentration of a predetermined analyte, such as glucose, cholesterol or occult blood, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an indicator reagent composition capable of interacting with the predetermined analyte to produce a detectable or measurable response. In addition, the new and improved indicator reagent composition, comprising an indicator dye, such as a redox indicator, like a benzidine indicator; a redox mediator, such as an oxidase enzyme capable of interacting with the predetermined analyte and a peroxidase enzyme; a metal ion complex; and a cooxidant selected from the group consisting of bromate ion, chlorate ion, perchlorate ion, chromate ion, an organic oxidant like a peroxide, a hydroperoxide or a N-halo compound, and combinations thereof, is incorporated into the carrier matrix to provide a more accurate, ascorbate interference-resistant assay of a test sample for the predetermined analyte. MS-1654

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