Aspergillus niger beta-glucosidase gene, protein and uses...

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 19/14 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 9/42 (2006.01) C12N 15/09 (2006.01) C12N 15/31 (2006.01) C12N 15/56 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12P 7/02 (2006.01)

Patent

CA 2392060

A polypeptide having .beta.-glucosidase enzymatic activity, a polynucleotide encoding the polypeptide, a nucleic acid constructs carrying the polynucleotide, transformed or infected cells, such as yeast cells, and transgenic organisms expressing the polynucleotide and various uses of the polypeptide, the polynucleotide, cells and/or organisms, including, producing a recombinant polypeptide having the .beta.-glucosidase enzymatic activity, increasing the level of aroma compounds in alcoholic beverages, as well as other fermentation products of plant material hydrolyzing cellobiose and thus increasing the level of fermentable glucose, increasing the production of alcohol, such as ethanol from plant material increasing the aroma released from a plant or a plant product, and hydrolysis or transglycosylation of glycosides

La présente invention concerne un polypeptide possédant une activité enzymatique de beta -glucosidase, un polynucléotide codant le polypeptide, un produit de synthèse d'acide nucléique portant le polynucléotide, des cellules transformées ou infectées telles que des cellules de levures, et des organismes transgéniques exprimant le polynucléotide précité. L'invention se rapporte aux diverses utilisations du polypeptide, du polynucléotide, des cellules et/ou organismes précités, y compris à la production d'un polypeptide recombiné possédant l'activité enzymatique du beta -glucosidase, à l'augmentation du niveau des composés aromatiques dans les boissons alcoolisées et autres produits de la fermentation de matières végétales, à l'hydrolyse du cellobiose et, par conséquent, à l'augmentation du niveau de glucose fermentable, à l'augmentation de la production d'alcool comme l'alcool éthylique à partir de matières végétales, à l'augmentation des arômes émanant d'une plante ou d'un produit végétal, et à l'hydrolyse ou la transglucosylation des glycosides.

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