Assay

G - Physics – 01 – N

Patent

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Details

G01N 33/68 (2006.01)

Patent

CA 2543464

The invention relates to a method of determining the amount or presence of antibodies to an immunogen in a blood sample comprising at least the steps of detecting the amount or presence of plasma antibodies or parts thereof and lymphocyte antibodies or parts thereof in said sample, wherein said plasma and lymphocyte antibodies are detected together or in separate assays and determination of their combined amount or presence determines the amount or presence of antibodies to said immunogen. Uses of the method in high throughput screening, screening of blood bank samples and in diagnosis are also provided.

L'invention concerne une méthode de détermination de la quantité ou de la présence d'anticorps par rapport à un immunogène dans un prélèvement sanguin, ladite méthode consistant au moins à détecter la quantité ou la présence d'anticorps plasmatiques, ou de parties de ceux-ci, et d'anticorps de lymphocytes, ou de parties de ceux-ci, dans ledit prélèvement, lesdits anticorps plasmatiques et lesdits anticorps de lymphocytes étant détectés ensemble ou dans des dosages séparés, la détermination de leur quantité ou présence combinées déterminant la quantité ou présence d'anticorps par rapport audit immunogène. L'invention concerne également les utilisations de cette méthode dans le criblage à haut rendement, le criblage de prélèvements de la banque du sang, et le diagnostic.

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