Assay and compositions for detection of bacillus anthracis...

Details Assay and compositions for detection of bacillus anthracis... Assay and compositions for detection of bacillus anthracis...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C12N 1/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2506151

The invention includes compositions and methods of detection of Bacillus anthracis that use oligonucleotide probes specific for genetic material contained in the pXO1 and pX02 plasmids in nucleic acid hybridization reactions. Embodiments of the method may include additional probes specific for other gene sequences to distinguish B. anthracis from other bacterial species present in a sample or to provide an indication that the assay was performed properly even when no Bacillus sequence is detected.

L'invention concerne des compositions et des procédés de détection de Bacillus anthraci faisant intervenir des échantillons d'oligonucléotides spécifiques aux matériaux génétiques contenus dans les plasmides pXO1 et pXO2 dans des réactions d'hybridation d'acides nucléiques. Dans certains modes de réalisation, les procédés selon l'invention peuvent faire intervenir des échantillons additionnels spécifiques à d'autres séquences génétiques afin de distinguer B. anthracis d'autres espèces bactériennes présentes dans un échantillon, ou d'indiquer que le dosage à été effectué correctement, même lorsqu'aucune séquence de Bacillus n'a été détectée.

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