Assay and kits for detecting protein binding

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2526847

The invention provides methods for determining the interactions between phage~- displayed proteins and test molecules. The phage-displayed proteins are contacted with a reference moiety in the presence and absence of a test molecule; the behavior of the phage-~displayed proteins as a function of concentration of the test molecule permits calculation of the binding affinity of the phage-displayed protein for the test molecule.

L'invention concerne des procédés servant à déterminer les interactions entre des protéines présentées à la surface de phages et des molécules test. Selon ce procédé, les protéines présentées à la surface de phages sont mises en contact avec une fraction de référence en présence d'une molécule test et en l'absence de cette dernière; le comportement des protéines présentées à la surface de phages en fonction de la concentration de la molécule test permet de calculer l'affinité de liaison de la protéine présentée à la surface des phages vis-à-vis de la molécule test.

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