Assay apparatus and method using microfluidic arrays

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/68 (2006.01) B01L 3/00 (2006.01) C12P 19/34 (2006.01) C40B 30/00 (2006.01) C40B 40/06 (2006.01) C40B 60/12 (2006.01) G01N 1/00 (2006.01) G01N 1/44 (2006.01) G01N 21/03 (2006.01) G01N 21/25 (2006.01) G01N 21/78 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2521999

A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers (22, 23), at least one of which is light transmissive, of which pair a light transmissive cover forms a top (22), and of which pair the other forms a bottom (23). A frame (21) is disposed between the covers to define, in relation to the covers, an interior volume (24). The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

L'invention concerne un système destiné à contenir au moins un échantillon et/ou un réactif à des fins d'analyse. Ce système comprend une paire de plaques parallèles dont l'une au moins transmet la lumière et forme une partie supérieure tandis que l'autre forme une partie inférieure. Un cadre est disposé entre les plaques afin de définir un volume intérieur par rapport aux plaques. Le cadre et les plaques sont associés les uns aux autres afin de former une cuve sensiblement étanche aux liquides. Un réseau microfluidique est disposé dans le volume intérieur. Ce réseau comprend une feuille de matériau présentant deux surfaces opposées, une épaisseur et une pluralité de trous traversant l'épaisseur entre les surfaces, ces trous traversants contenant un échantillon et/ou un réactif.

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