Assay based on doped nanoparticles

Details Assay based on doped nanoparticles Assay based on doped nanoparticles

G - Physics

01

N

G01N 21/64 (2006.01) B82B 1/00 (2006.01) C12Q 1/34 (2006.01) C12Q 1/37 (2006.01) C12Q 1/42 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 21/78 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01) G01N 33/60 (2006.01)

Patent

CA 2465646

The invention relates to an assay based on resonance energy transfer (RET), comprising a first molecule group A, which is marked with at least one energy donor, and at least one second molecule group B which is marked with at least one energy acceptor, the donor comprising a molecule or particle which can be energetically excited by an external radiation source and which is fluorescence enabled and the acceptor comprising a molecule or particle which can be excited by energy transfer via the donor with partial or complete quenching of the donor fluorescence, and the donor and/or acceptor comprise luminescing inorganic doped nanoparticles having an expansion of <= 50 nanometers, emitting electromagnetic radiation with stokes or anti-stokes scattering after energetic excitation.

L'invention concerne une analyse reposant sur le transfert d'énergie de résonance (RET), faisant intervenir un premier groupe de molécules (A) marqué au moyen d'au moins un donneur d'énergie, et au moins un deuxième groupe de molécules (B) marqué au moyen d'un moins un accepteur d'énergie. Selon l'invention, ledit donneur comporte une molécule ou des particules fluorescentes pouvant être excitées énergétiquement au moyen d'une source de rayonnement extérieure, l'accepteur comporte une molécule ou des particules pouvant être excitées par transfert d'énergie par l'intermédiaire du donneur, avec suppression partielle ou complète de la fluorescence du donneur, et le donneur et/ou l'accepteur comportent des nanoparticules dopées, inorganiques, luminescentes, présentant une expansion <= 50 nanomètres, lesdites nanoparticules émettant un rayonnement électromagnétique avec un décalage de Stokes ou anti-Stokes après excitation énergétique.

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