G - Physics
01
N
G01N 33/558 (2006.01) B01L 3/00 (2006.01) G01N 30/90 (2006.01) G01N 33/53 (2006.01) G01N 33/72 (2006.01) G01N 30/02 (2006.01) G01N 30/30 (2006.01) G01N 37/00 (2006.01)
Patent
CA 2103052
A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels, The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (1) a first opposable component (12) comprising a sample preparation means (16) adapted to receive a sample to be assayed; and (2) a second opposable component (14) comprising a chromatographic medium (18). The first and second opposable components can be brought into opposition so as to cause the sample preparation means to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analyzes, as well as to permit bi-directional chromatography, The devices can be incorporated in test kits, and assay methods using the devices are also disclosed.
Macrae & Co.
Smithkline Diagnostics Inc.
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