Assay device and method

G - Physics – 01 – N

Patent

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Details

G01N 33/558 (2006.01) B01L 3/00 (2006.01)

Patent

CA 2654928

There is provided an assay device comprising a lid and a base, said base comprising, a sample addition zone, a reaction zone and an absorbing zone, said components being in fluid connection and being part of a fluid passage leading from the sample addition zone to the absorbing zone, wherein: (a) a sample addition well is integrated in the lid, (b) the absorbing zone consists of an area on an non-porous substrate, having substantially perpendicular projections, said projections defining a volume, which together with the volume of the fluid passage defines the sample volume subjected to the assay, and (c) at least one filter is between the sample addition well and the sample addition zone. There is further provided methods for handling samples.

La présente invention concerne un dispositif de dosage qui comprend un couvercle et une base. Ladite base se compose d'une zone d'ajout d'échantillon, d'une zone de réaction et d'une zone d'absorption et lesdits composants se trouvent en communication fluide et font partie d'un passage fluide menant de la zone d'ajout d'échantillon à la zone d'absorption. Dans ledit dispositif : (a) un puits d'ajout d'échantillon est incorporé au couvercle ; (b) la zone d'absorption correspond à une surface de substrat non poreux, dotée de protubérances sensiblement perpendiculaires, lesdites protubérances définissant un volume qui définit avec le volume du passage du fluide le volume de l'échantillon soumis au dosage ; et (c) un filtre au moins est placé entre le puits d'ajout d'échantillon et la zone d'ajout d'échantillon. L'invention se rapporte aussi à des procédés de manipulation d'échantillon.

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