C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/18 (2006.01) C12Q 1/34 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01)
Patent
CA 2076021
ASSAY DEVICE AND METHOD OF DETECTING CHITIN Abstract of the Invention A method for detecting chitin-containing organisms such as fungi, yeast and insects in a sample attached to a solid phase, by detecting the presence of chitin by using enzymes which specifically bind chitin is disclosed. The method is preferably carried out by passing a sample which contains chitin through a membrane in order to capture and adhere the chitinous material on the membrane. A chitin-binding enzyme such as chitinase or lysozyme is added under conditions which allow it to bind to the chitin on the membrane. A signal generating compound is either directly conjugated to the chitinase (or lysozyme) or indirectly conjugated to either an anti- chitinase (or lysozyme) antibody or to an anti IgG antibody (which binds to the anti-chitinase antibody) to detect the chitin-chitinase complex. Reagents for the signal generating compound then are added to produce a detection signal which indicates the presence of the chitin. Kits which contain the necessary materials for carrying out the methods of the invention are also disclosed as are novel conjugates.
Dousman Linda
Tuse Daniel
Gowling Lafleur Henderson Llp
Sri International
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