C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
324/23
C12M 1/40 (2006.01) G01N 27/30 (2006.01) G01N 27/42 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/76 (2006.01)
Patent
CA 1312650
A B S T R A C T : The invention relates to a novel assay for the deter- mination of entities having biological activity and to a device for carrying out such determinations. The assay is a very sensitive one and quantities in the picogram per millimeter range can be determined. The assay is based on chrono-coulometric measurements with sequential measurements of a plurality of samples being carried out with the aid of a multiplexer, in combination with a potentiostat, there being provided suitable electrodes and means for applying a predeter- mined voltage. One of the electrodes is advantageously a glassy carbon electrode, carbon felt or cloth or carbon paper. Amongst biologically active entities there are antibodies/antigens; hormones/receptors ; nuclotides/ nucleotide probes, one of the members of such a pair being immobilized on an electrode surface, being tagged with an enzyme providing a signal in coulo- metric measurements.
563191
Rishpon Judith
Rosen Ilana
Fetherstonhaugh & Co.
Ramot University Authority For Applied Research And Industrial D
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