Assay device that analyzes the absorption, metabolism,...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/14 (2006.01) C12M 1/00 (2006.01) C12M 1/42 (2006.01) C12Q 1/02 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2479072

This invention provides device for co-culturing at least two different cell types in a two-dimensional configuration, methods of patterning at least two different cell types in a two-dimensional co-culture configuration, and uses of these devices and methods for analyzing an effect of candidate compound on such cellular co-cultures. Also provided is a transmigration and extravasation device. Assay devices for analyzing the absorption, permeability, metabolism and/or toxicity of a candidate compound by a cell are provided. A microfluidic network, which is adaptable for integration with a device for coculturing is provided.

L'invention concerne un dispositif destiné à la co-culture d'au moins deux types cellulaires différents dans une configuration bidimensionnelle, des procédés permettant de disposer au moins deux types cellulaires différents dans une configuration de co-culture bidimensionnelle, ainsi que l'utilisation de ces dispositifs et de ces procédés pour analyser l'effet d'un composé étudié sur ces co-cultures cellulaires. L'invention concerne également un dispositif de transmigration et d'extravasation. L'invention concerne également des dispositifs de dosage permettant d'analyser l'absorption, la perméabilité, le métabolisme et/ou la toxicité d'un composé étudié par rapport à une cellule. L'invention concerne également un réseau microfluidique pouvant être adapté pour être intégré dans un dispositif de co-culture.

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