Assay device with gradient-regulated localized signal

Details Assay device with gradient-regulated localized signal Assay device with gradient-regulated localized signal

G - Physics

01

N

150/11, 150/14,

G01N 33/543 (2006.01) C12M 1/34 (2006.01) G01N 33/52 (2006.01) G01N 33/542 (2006.01) G01N 33/559 (2006.01) G01N 33/58 (2006.01) G01N 33/92 (2006.01)

Patent

CA 1338734

Disclosed is a device for detecting an analyte in a sample, which device comprises a reaction matrix containing a solution and signal localization medium in which a signal can be generated from the analyte or analyte derivative in said solution unbound to said localization medium and convection and diffusion of any such signal in said solution is diminished by the effect ofsaid localization medium on said solution; a gradient, combined or in contact with at least said signal localization portion of said matrix, of a species which is reactive with said analyte or analyte derivative to regulate production of signal therefrom; and a composition which when exposed to any remaining analyte or analyte derivative generates a detectable signal therefrom.

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