Assay devices using subsurface flow

Details Assay devices using subsurface flow Assay devices using subsurface flow

G - Physics

01

N

G01N 33/558 (2006.01) B01L 3/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2144976

The present invention is directed to improving the performance of assays using a disposable assay device which includes a porous material in liquid communication with a capillary track. In particular, the capillary track is used in conjunction with the solid support to direct test sample and assay reagents directly to a defined reaction site on or in the porous material. Signal devel- opment at the reaction site indicates the assay result. The present invention is also directed to the construction of a disposable as- say device which includes a capillary track. In particular, the cappillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a film layer and then capping the printed material. Alternatively, the capillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a porous material which is then sandwiched between two film layers.

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