Assay electrodes having immobilized lipid/protein layers,...

G - Physics – 01 – N

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G01N 33/92 (2006.01) C12N 11/02 (2006.01) C12N 11/06 (2006.01) C12Q 1/00 (2006.01) C40B 40/04 (2006.01) C40B 50/14 (2006.01) C40B 60/12 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2455932

The present invention includes improved systems, system components, and methods for performing assays involving biological membranes and/or components thereof. Such systems are preferably compatible with a multi-well plate assay format. The invention also relates to solid phase supports in the form of electrodes (Figure 1E) having biological membranes, membrane proteins, and/or lipid/protein layers immobilized thereon and method of making and using the same. The assays of the invention are preferably coupled to a detection step that involves the use of an electrode, the generation of light, and the measurement of the generated light.

L'invention concerne des systèmes améliorés, des composants de système ainsi que des procédés de réalisation de tests mettant en oeuvre des membranes biologiques et/ou des composants de ces membranes. De tels systèmes sont de préférence compatibles avec un format de test par plaque à puits multiples. L'invention concerne aussi des supports de phase solide sous la forme d'électrodes (Figure 1E) comportant des membranes biologiques, des protéines de membrane, et/ou des couches de protéine/lipide immobilisés sur ces électrodes ainsi que des procédés de fabrication et d'utilisation de ces supports. Les tests de l'invention sont, de préférence, couplés à une étape de détection mettant en oeuvre une électrode, la production de rayonnement lumineux et la mesure du rayonnement lumineux produit.

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