Assay employing both protein and antibody

Details Assay employing both protein and antibody Assay employing both protein and antibody

G - Physics

01

N

G01N 33/566 (2006.01) G01N 33/541 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2180428

The binding site on immunoglobulin for a protein is blocked by hydrophobically coupling the immunoglobulin to a blocking agent such as a label. This results in a detection reagent useful in a variety of test assays in which a protein and an immunoglobulin are advantageously used together, but separately. The reagent is useful in the simultaneous determination of IgG and one of IgA or IgM. Anti-IgA-IgG or anti-IgM-IgG is coupled to a hydrophobic label, particularly a pigment or dye, which label blocks the binding site on IgG for Protein A, and labeled Protein A is added.

Blocage du site de fixation sur une immunoglobuline pour une protéine par couplage hydrophobe de l'immunoglobuline à un agent de blocage tel qu'un marqueur. On obtient ainsi un réactif de détection servant dans différents tests dans lesquels une immunoglobuline et une protéine sont utilisées avantageusement l'une et l'autre, mais séparément. Ledit réactif sert à l'identification simultanée de l'IgG, et de l'IgA ou de l'IgM. Un anti IgA-IgG ou un anti IgM-IgG est couplé à un marqueur hydrophobe, en particulier à un pigment ou à un colorant, ledit marqueur bloquant le site de fixation sur l'IgG pour la protéine A, et la protéine A marquée étant ajoutée.

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