Assay for analysis of whole blood

G - Physics – 01 – N

Patent

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150/11, 150/15,

G01N 33/66 (2006.01) C12Q 1/26 (2006.01) G01N 33/52 (2006.01) G01N 33/92 (2006.01)

Patent

CA 1212602

-0- ASSAY FOR ANALYSIS OF WHOLE BLOOD Abstract of the Disclosure Disclosed herein is an assay useful for the determination of an analyte in whole blood. In particular, this assay is useful for the quantitative determination of peroxide-generating analytes, such as glucose or cholesterol, in whole blood. This assay utilizes a multizone element consisting essentially of a support having thereon, in order and in fluid contact, a registration zone and a reagent/spreading zone. The reagent/spreading zone has a void volume and average pore size effective to accomodate whole blood, and contains an interactive composition necessary for the analysis. Such composition is capable of providing, upon interaction with the analyte, a dye which can be spectrophotometrically detected at a wavelength greater than about 600 nm.

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