Assay for anti-ingap antibodies

G - Physics – 01 – N

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G01N 33/543 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2476979

A solid phase assay is used for detecting antibodies to INGAP104-118 peptide, a 15-amino acid peptide that is the biologically active portion of islet neogenesis associated protein (INGAP). The isotype of the antibodies to INGAP104-118 peptide can be determined. A kit can also be used in the detection of anti-INGAP104-118 antibodies. Endogenous autoantibodies or antibody production during therapeutic treatment of a mammal with INGAP104-118 can be monitored.

L'invention concerne un test en phase solide utilisé afin de détecter des anticorps contre le peptide INGAP?104-118¿, un peptide de 15 acides aminés qui constitue la partie biologiquement active de la protéine associée à la néogenèse des îlots de Langerhans (INGAP). L'isotype des anticorps dirigés contre le peptide INGAP?104-118¿ peut être déterminé. On peut aussi utiliser un kit de détection des anticorps anti-INGAP?104-118¿. Il est ainsi possible de suivre la production d'autoanticorps endogènes ou d'anticorps lors d'un traitement thérapeutique d'un mammifère avec le peptide INGAP?104-118¿.

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