Assay for asymmetrical dimethyl-l-arginine (adma)

Details Assay for asymmetrical dimethyl-l-arginine (adma) Assay for asymmetrical dimethyl-l-arginine (adma)

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01)

Patent

CA 2327413

ADMA is determined in a physiological sample by first removing interfering components: proteins by precipitation; and amines and citrulline, by means of a cation exchange column, followed by enzymatic hydrolysis of the ADMA to citrulline with DDAH. The citrulline is then determined spectrophotometrically.

On détermine la teneur en diméthyl-L-arginine asymétrique (ADMA) d'un échantillon physiologique en éliminant tout d'abord les composants perturbateurs ci-après: les protéines par précipitation ; et les amines et la citrulline au moyen d'une colonne d'échange de cations, puis par hydrolyse enzymatique de la diméthyl-L-arginine asymétrique en citrulline à l'aide de DDAH. On détermine ensuite la teneur en citrulline par spectrophotométrie.

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