Assay for detecting fibrinolysis and figrinogenolysis based...

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G01N 33/577 (2006.01) G01N 1/34 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2112228

2112228 9300364 PCTABS00019 This invention provides a method of detecting the presence of an A.alpha. fragment in a fibrinogen- or fibrinogen derivative-containing sample from a human subject which comprises: contacting the sample with an agent capable of forming a complex with intact fibrinogen, or with a fibrinogen derivative which contains an A.alpha. chain under conditions permitting formation of a complex; removing a complex so formed from the sample; contacting the resulting sample with a monoclonal antibody capable of forming a complex with an epitope containing amino acid residues present exclusively between amino acid residue 477 and the carboxy terminus of the A.alpha. fragment, under conditions permitting formation of a monoclonal antibody A.alpha. fragment complex; and detecting any such complex formed in the sample, thereby detecting the presence of A.alpha. fragment in the sample.

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