Assay for enzyme activity

Details Assay for enzyme activity Assay for enzyme activity

C - Chemistry, Metallurgy

12

Q

C12Q 1/44 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2065410

2065410 9104338 PCTABS00003 A method of assay for an enzyme catalysing the release of long chain fatty acids or, conversely, for an ester substrate for such enzymes is applicable to clinical samples and comprises removing substantially all the albumin from the clinical sample, incubating the sample after albumin removal with, for enzyme assay, an ester substrate or, for substrate assay, the enzyme, under conditions effective to release a fatty acid, causing the fatty acid to bind to a fatty acid binding protein and assaying the binding of the fatty acid to the binding protein.

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