Assay for gene expression

Details Assay for gene expression Assay for gene expression

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2680426

An assay for gene expression comprising treating RNA with an agent such as bisulphate that substantially removes secondary structure of the RNA; and measuring the presence or amount of treated RNA so as to obtain an indication of gene expression. The invention also includes use of oligonucleotide, PNA, LNA or INA probes in the assay.

Cette invention a trait à un dosage de l'expression génétique comprenant les étapes consistant à traiter de l'ARN avec un agent tel qu'un bisulfate qui élimine sensiblement la structure secondaire de l'ARN; puis à mesurer la présence ou la quantité d'ARN traité de manière à obtenir une indication de l'expression génétique. L'invention concerne également l'utilisation de sondes oligonucléotidiques, PNA, LNA ou INA dans le dosage.

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