Assay for identifying antibody producing cells

G - Physics – 01 – N

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Details

G01N 33/544 (2006.01) C12N 5/078 (2010.01) C07K 16/00 (2006.01) G01N 33/52 (2006.01) G01N 33/554 (2006.01) C12P 21/00 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2507004

The present invention provides a homogeneous assay for identifying an antibody producing cell producing an antibody which binds to a selected antigen comprising: a) providing a population of antibody producing cells; b) incubating said population of antibody producing cells with a selected antigen and a labeled anti-antibody antibody, wherein said anti-antibody antibody is capable of distinguishing cells producing an antibody which binds to the selected antigen from those cells which do not; and c) identifying an antibody producing cell capable of producing an antibody which binds to the selected antigen.

La présente invention concerne un dosage biologique homogène permettant d'identifier une cellule productrice d'anticorps, laquelle cellule produit un anticorps qui se lie à un antigène sélectionné. Le dosage biologique décrit dans cette invention consiste: a) à fournir une population de cellules productrices d'anticorps; b) à procéder à l'incubation de cette population avec un antigène sélectionné et avec un anticorps anti-anticorps marqué, lequel est capable de distinguer des cellules produisant un anticorps qui se lie à l'antigène sélectionné des autres cellules; et c) à identifier une cellule productrice d'anticorps capable de produire un anticorps qui se lie à l'antigène sélectionné.

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