Assay for interferon epsilon

Details Assay for interferon epsilon Assay for interferon epsilon

G - Physics

01

N

167/45

G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1244345

ASSAY FOR INTERFERON EPSILON Abstract of the Disclosure Disclosed is an assay procedure for detecting and quantifying interferon epsilon, a new composition of matter having selective antiviral activity on epithelial cells. The assay comprises incubating a preparation believed to contain interferon epsilon with human keratinocyte cells and with human fibroblast cells followed by a virus challenge. The presence of interferon epsilon is indicated if the preparation has antiviral activity on the keratinocytes but no detectable activity on the fibroblasts. The titer of the preparation may be determined by serially diluting it, incubating the dilution with subcultures of keratinocytes, challenging the subcultures with a virus, observing the viability of cells in the cultures, and comparing the results with a standard.

473754

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