Assay for ligands of the ecdysone receptor

C - Chemistry – Metallurgy – 07 – J

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C07J 31/00 (2006.01) C07J 41/00 (2006.01)

Patent

CA 2547609

The present invention provides an ecdysteroid derivative in which a fluorescent moiety is attached to an ecdysteroid moiety by derivatisation of a hydroxyl group on the alkyl side chain of the ecdysteroid moiety. The derivative is capable of binding to an ecdysone receptor or ligand binding domain thereof and can be used in assays.

La présente invention concerne un dérivé d'ecdystéroïde dans lequel une fraction fluorescente est attachée à une fraction d'ecdystéroïde par dérivation d'un groupe hydroxyle sur la chaîne latérale alkyle de la fraction d'ecdystéroïde. Ce dérivé peut se lier à un récepteur d'ecdysone ou à un domaine de liaison aux ligands et peut être utilisé dans des essais biologiques.

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