Assay for measuring factor viia-antithrombin complexes

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/37 (2006.01) C07K 16/40 (2006.01) C12Q 1/56 (2006.01) G01N 33/543 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2451378

A method for determining the concentration of factor VIIa-antithrombin complexes is disclosed which has application to estimating the level of intravascular exposure of tissue factor, assessing patient risk for hypercoagulation or other coagulopathies, and monitoring patients for factor VIIa-antithrombin complexes over time which can reveal changes in risk for hypercoagulation or other coagulopathies and/or effectiveness of anticoagulant therapy. Antibodies suitable for use in an in vitro assay for determining the concentration of factor VIIa-antithrombin complexes and methods for making the same are also disclosed.

L'invention concerne une méthode destinée à déterminer la concentration de complexes facteur VIIa-antithrombine et permettant d'estimer le niveau d'exposition intravasculaire du facteur tissulaire, d'évaluer le risque pour un patient de développer une hypercoagulation ou d'autres coagulopathies, et de surveiller la concentration des complexes facteur VIIa-antithrombine chez des patients au fil du temps, d'où la possibilité de détecter des changements du niveau de risque d'hypercoagulation ou d'autres coagulopathies et/ou de l'efficacité d'une thérapie anticoagulante. L'invention concerne également des anticorps destinés à des analyses in vitro et servant à déterminer la concentration des complexes facteur VIIa-antithrombine, ainsi que leurs méthodes de fabrication.

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