Assay for nucleic acid sequences, particularly genetic lesions

Details Assay for nucleic acid sequences, particularly genetic lesions Assay for nucleic acid sequences, particularly genetic lesions

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) C12Q 1/00 (2006.01) G01N 33/58 (2006.01) H01R 13/68 (2006.01) H01R 13/74 (2006.01)

Patent

CA 1258029

Abstract Lesions in genetic sequences, for example, the sickle cell anemia mutation in the beta globin gene, are detected by means of interactive labels in a nucleic acid hybridization assay. A signal is generated only if the labels are in physical proximity, which is made dependent on whether a predetermined normal or abnormal sequence is present by exposure to appropriate conditions such as restriction enzyme digestion or stringent hybridization.

496059

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