Assay for nucleic acids

Details Assay for nucleic acids Assay for nucleic acids

G - Physics

01

N

150/15, 324/23

G01N 33/48 (2006.01) C12M 1/40 (2006.01) C12Q 1/00 (2006.01) C12Q 1/54 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1223639

A B S T R A T ASSAY FOR NUCLEI ACIDS The present invention discloses an assay for nucleic acid which comprises the steps of; (a) providing a probe material comprising; (i) a sequence of nucleic acids complementary to a given target sequence and, (ii) a first ligand chemically linked thereto and capable of a specific binding reaction with an antiligand; (b) contacting the said probe material with an assay system comprising; (i) a suitable mediator, enzyme, substrate system capable of transferring charge to an electrode surface when the enzyme is catalytically active, and; (ii)a second ligand chemically linked to one of said mediator, enzyme or substrate, wherein the second ligand is capable of a competitive binding reaction with the antiligand, and; (iii) the said antiligand, whereby the said first ligand completes with the said second ligand specific in a specific binding reaction with the antiligand, and; (c) contacting the above system with a solution suspected of containing the said target sequence whereby the binding of any of the said target sequence present to the probe affects the availability of the first ligand and therefore alters the rate of charge transfer to the electrode. Refer to Figure 1

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