Assay for salicylate and apparatus for performing same

Details Assay for salicylate and apparatus for performing same Assay for salicylate and apparatus for performing same

C - Chemistry, Metallurgy

12

Q

324/23

C12Q 1/26 (2006.01) C12N 9/02 (2006.01) G01N 27/48 (2006.01)

Patent

CA 1253568

A B S T R A C T The specification discloses a method and apparatus for the assay of salicylate (or a derivative thereof) comprising a conducting body having at a surface thereof an enzyme capable of catalysing the conversion of salicylate (or a derivative thereof) to a catechol whereby said catechol is subject to direct electrochemical measurement as it oxidises at said surface to generate a current as a measure of the reaction taking place and thereby of the concentration of salicylate at said surface. The method typically comprises the steps of; a)treating a liquid sample suspected of containing salicylate or a derivative thereof with an enzyme capable of catalysing the conversion of salicylate or a derivative thereof into a catechol, and, b) measuring the concentration of catechol in the treated sample by direct electrochemistry.

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