Assay for the detection of factors that modulate the...

A - Human Necessities – 61 – K

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Details

A61K 38/24 (2006.01) A61K 38/27 (2006.01) C07K 1/00 (2006.01) C07K 14/00 (2006.01) C07K 14/47 (2006.01) C07K 16/00 (2006.01) C07K 17/00 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12P 21/06 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2470359

A reporter construct contains mammalian INGAP 5'-regulatory region or a fragment thereof, a minimal promoter element from mammalian INGAP or a heterologous promoter, and a reporter gene. The reporter construct can be used to screen for agents which alone or in combination up-regulate or down- regulate reporter gene expression. Alternatively, the reporter construct can be used to screen for agents that bind to the hamster INGAP 5'-regulatory region or a fragment thereof.

Cette invention concerne une construction reporter contenant une région régulatrice INGAP 5' de mammifère ou un fragment de cette région, un élément promoteur minimal pour le gène de mammifère ou un promoteur hétérologue, et un gène rapporteur. Cette construction rapporteur peut s'utiliser pour cribler des agents qui, seuls ou associés, augmentent ou réduisent la régulation de l'expression du gène rapporteur. En variante, la construction rapporteur peut être utilisée pour cribler des agents qui se lient à la région régulatrice d'INGAP 5' chez le hamster ou à un fragment de cette région.

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