G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/558 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2110920
2110920 9222797 PCTABS00018 The invention disclosed herein relates to the field of one-step assays and particle agglutination tests. In one embodiment of the present invention, there is disclosed an analytical device for the detection or determination of an analyte antibody in a bodily fluid comprising a layer of a plurality of substantially planar zones adjacent one another and in absorbent contact with one another, the layer including a sample application zone, a conjugate zone containing antigen bound to mobile particles, and a detection zone containing immobilized antigen, wherein the antigen is the same in both the conjugate and detection zones and is an antigen that binds with the analyte antibody, the liquid sample is capable of moving from the sample application zone through the conjugate zone and on to the detection zone, and if the analyte antibody is present in the sample it is detected in the detection zone. The present invention also discloses methods of detecting various analytes, particularly analyte antibodies, including specific immunoglobulins. The invention further provides improved methods of preparing and using one-step assays, as well as improved methods of preparing coated particles for use in diagnostic assays.
Tzeng Sinfu
Wang Dou-Mei
Pacific Biotech Inc.
Sim & Mcburney
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