Assay for the determination of ester substrates

Details Assay for the determination of ester substrates Assay for the determination of ester substrates

C - Chemistry, Metallurgy

12

Q

C12Q 1/61 (2006.01) C12Q 1/44 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2105871

2105871 9216847 PCTABS00016 A method of quantitative assay for a substrate such as triglyceride capable of undergoing enzymatic hydrolysis to release long chain fatty acids in an albumin-containing clinical sample comprises incubating the albumin-containing clinical sample with an enzyme such as lipase which acts upon the substrate to be assayed under conditions effective to release fatty acid therefrom, causing the fatty acid thus released to bind to a fatty acid binding protein (FABP) and assaying the binding of the fatty acid to the FABP.

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