Assay for unbound hormones and pharmaceuticals using a...

G - Physics – 01 – N

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167/41

G01N 23/00 (2006.01) G01N 33/74 (2006.01)

Patent

CA 1108531

ABSTRACT OF THE DISCLOSURE Disclosed is a method for the determination of unbound hormones and pharmaceuticals comprising placing a solution containing unbound hormones, hormones bound to bonding proteins and bonding proteins into contact with an immobilized antibody; reacting the unbound hormone with the antibody; eluting the hormones bound to bonding proteins and the bonding hormones with a solution contain- ing a marked hormone; reacting the marked hormone with the antibody; eluting the marked hormone not reacted with the antibody, and determining the marked hormone by radioimmunological evaluation.

302084

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